In my opinion if you want to rule out HIV go for the new PCR viral load test down to 20 copies/ml (ultra-sensative HIV-1 RNA by PCR) recently passed by the FDA. If the results are clean than there is no reason to be obscene unless you are 1 in a million with less than 20 HIV-1 copies m/l. It's like winning the lottery; but you never know you might be that unlucky person. Two years ago the FDA passed the HIV-1 RNA by PCR test which measures HIV-1 down to 400 RNA copies m/l. My guess it will be another two years before they pass the ultra ultra sensative HIV-1 RNA copies m/l that will measure down to 1 copy m/l for 100.00000000% specificity. Remember, it only takes 1 copy of HIV-1 RNA to duplicate over time to do damage to kill a person!!! My question is to Dr. Holidniy. I have not tested positive to any HIV-1 antibody tests only negatives (5 serum ELISA) and indeterminate (2 serum WESTERN BLOT) but low positives on (3 PCR HIV1-RNA viral load tests). You previously recommended in my case that I should go for a HIV-1 DNA viral load test. My doctor did not know anything about this test. He told me that the virus is RNA not DNA. Could you please explicate this to me so I can educate my doctor.
Also I tested negative to a HIV-1 p24 antegen test. Is it true that only a p24 HIV band will trigger the HIV-1 ELISA antibody to be positive? Is this the first band that normally develops when exposed to HIV-1 but not always like my case? I have weak reactivity at p51 and p56 and unusual weak reactivity in the area of 75,000 molecular weight. Can you tell me what this really means? I am so confused and feeling horrible. Thank you for your help.
HIV is an RNA virus, but once it gets inside cells, it is converted to DNA by the HIV reverse transcriptase enzyme. In about 100% of people, the p24 antibody is the first to appear and will be the first band positive on the western blot. Have you had a subsequent western blot to see if more or other bands are positive. Have you had an HIV-2 antibody test? You may be one of the few who has a false positive western blot.