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Will A Dna Pcr Detect All Hiv Subtypes

Here's a taste of what TheBody.com has to offer on this topic:
Ask the Experts

HIV DNA PCR and Subtypes

Dear Doctor Holodniy:I understand that the RT-PCR viral load test may miss subtype A. What about the DNA PCR test? Will it detect proviral dna associated with all HIV-1 subtypes? Thank you for your time....
Mark Holodniy, M.D., F.A.C.P., C.I.C.

Response from Mark Holodniy, M.D., F.A.C.P., C.I.C.

Stanford University, School of Medicine
The current version of the HIV-1 Monitor test which uses RT-PCR may miss, or detect but be unable to quantitate subtype A plasma RNA. Modifications to PCR gene primers have been made and are currently being tested which allow for this detection. The... Read more »

DNA PCR missing some subtypes?

I have read on this forum and on various online articles that the Qualitative DNA PCR can miss... Read more »

DNA PCR - Concern about Rare Subtypes

Hi, Richard. I had a DNA PCR done at my doctor's office in 2008. Would this have detected all... Read more »
Articles
HIV i-Base

Recombination Dynamics in Case of MDR HIV Sub-Type D Following Superinfection With Wild-Type HIV Sub-Type B

While research into reinfection is largely driven by expanding number of case studies, Koning and colleagues from the UK described the dynamics of reinfection with wild-type virus, using single genome sequencing (SGS) in both plasma and semen... Read more »
U.S. Centers for Disease Control and Prevention

Type of AIDS Infection Key to Death Risk -- Study

The clade, or subtype, of HIV infection was a better predictor of rapid AIDS mortality than viral load in a Ugandan study recently presented at the 13th Conference on Retroviruses and Opportunistic Infections in Denver. Conducted by US and Ugandan... Read more »
U.S. Centers for Disease Control and Prevention

Portable Device Detects HIV With Push of a Button

After approximately 10 years of work, researchers at the California Institute of Technology (Caltech) have built an inexpensive, portable, easy-to-use device to quickly diagnose HIV/AIDS and other diseases. Axel Scherer and George Maltezos began... Read more »
TheBodyPRO.com

Detectable HIV May Be in Semen Despite Viral Suppression in Blood

HIV can still be detected in the semen of some HIV-infected men, even if they are on sustained antiretroviral therapy and have an undetectable viral load in their blood, according to a study presented at IAS 2013 . The study, conducted by French... Read more »
Connect With Others

PCR not detecting all strains of HIV?

Posted by Anonymous, 1 Reply
As many worried persons, I took a PCR-RNA 4 weeks after my infection risk, to amend my fears. It came back negative. Although I should relax now, I'm still concerned, because I have read that all commercially available PCR-kits are just looking for... Read more »

PCR and subtypes

Posted by Anonymous
Hi,

Can anyone tell me if they have heard of HIV-1 Proviral DNA PCR test. The kit used for the test is artus RealArt RG RT-PCR.
Does anyone know if it covers all the major subtypes ..i.e A, B, C, D, E, F& G. I tried calling the concellor at th... Read more »

PCR and subtypes

Posted by Anonymous
Hi,

Can anyone tell me if they have heard of HIV-1 Proviral DNA PCR test. The kit used for the test is artus RealArt RG RT-PCR.
Does anyone know if it covers all the major subtypes ..i.e A, B, C, D, E, F& G. I tried calling the concellor at th... Read more »

DNA PCR HIV

Posted by jade2008, 1 Reply
. Can someone please tackle this? Point is, if the DNA PCR test can determine the presence of HIV as soon as the symptoms of ARS are felt, then it may make some sense to counsel people who are going out of their minds waiting 3 months for a test and... Read more »