Will A Dna Pcr Detect All Hiv Subtypes

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Ask the Experts

HIV DNA PCR and Subtypes

Dear Doctor Holodniy:I understand that the RT-PCR viral load test may miss subtype A. What about the DNA PCR test? Will it detect proviral dna associated with all HIV-1 subtypes? Thank you for your time....

Response from Mark Holodniy, M.D., F.A.C.P., C.I.C.

Stanford University, School of Medicine

The current version of the HIV-1 Monitor test which uses RT-PCR may miss, or detect but be unable to quantitate subtype A plasma RNA. Modifications to PCR gene primers have been made and are currently being tested which allow for this detection. The... Read more »

DNA PCR missing some subtypes?

I have read on this forum and on various online articles that the Qualitative DNA PCR can miss... Read more »

Response from Mark Holodniy, M.D., F.A.C.P., C.I.C. »

DNA PCR - Concern about Rare Subtypes

Hi, Richard. I had a DNA PCR done at my doctor's office in 2008. Would this have detected all... Read more »

Response from Richard Cordova III »

Go to Ask the Experts »

Articles

Recombination Dynamics in Case of MDR HIV Sub-Type D Following Superinfection With Wild-Type HIV Sub-Type B

While research into reinfection is largely driven by expanding number of case studies, Koning and colleagues from the UK described the dynamics of reinfection with wild-type virus, using single genome sequencing (SGS) in both plasma and semen... Read more »

U.S. Centers for Disease Control and Prevention

Type of AIDS Infection Key to Death Risk -- Study

The clade, or subtype, of HIV infection was a better predictor of rapid AIDS mortality than viral load in a Ugandan study recently presented at the 13th Conference on Retroviruses and Opportunistic Infections in Denver. Conducted by US and Ugandan... Read more »

Portable Device Detects HIV With Push of a Button

After approximately 10 years of work, researchers at the California Institute of Technology (Caltech) have built an inexpensive, portable, easy-to-use device to quickly diagnose HIV/AIDS and other diseases. Axel Scherer and George Maltezos began... Read more »

PLoS Medicine, Journal of Infectious Diseases Articles on HIV/AIDS, Global Health Issues; Uncommon HIV Subtypes in Minnesota; 2005 Gates Award for Global Health

PLoS Medicine : The May 31 issue of the journal includes several articles and studies on HIV/AIDS and other global health issues, including "HIV Epidemiology in Africa: Weak Variables and Tendentiousness Generate Wobbly Conclusions ," "The... Read more »

Connect With Others

PCR not detecting all strains of HIV?

Posted by Anonymous, 1 Reply

As many worried persons, I took a PCR-RNA 4 weeks after my infection risk, to amend my fears. It came back negative. Although I should relax now, I'm still concerned, because I have read that all commercially available PCR-kits are just looking for... Read more »

PCR and subtypes

Posted by Anonymous

Hi,

Can anyone tell me if they have heard of HIV-1 Proviral DNA PCR test. The kit used for the test is artus RealArt RG RT-PCR.
Does anyone know if it covers all the major subtypes ..i.e A, B, C, D, E, F& G. I tried calling the concellor at th... Read more »

PCR and subtypes

Posted by Anonymous

DNA PCR HIV

Posted by jade2008, 1 Reply

. Can someone please tackle this? Point is, if the DNA PCR test can determine the presence of HIV as soon as the symptoms of ARS are felt, then it may make some sense to counsel people who are going out of their minds waiting 3 months for a test and... Read more »

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