Advertisement

The Body: The Complete HIV/AIDS Resource
Follow Us Follow Us on Facebook Follow Us on Twitter Download Our App
Professionals >> Visit The Body PROThe Body en Espanol

Will A Dna Pcr Detect All Hiv Subtypes

Here's a taste of what TheBody.com has to offer on this topic:
Ask the Experts

HIV DNA PCR and Subtypes

Dear Doctor Holodniy:I understand that the RT-PCR viral load test may miss subtype A. What about the DNA PCR test? Will it detect proviral dna associated with all HIV-1 subtypes? Thank you for your time.
Mark Holodniy, M.D., F.A.C.P., C.I.C.

Response from Mark Holodniy, M.D., F.A.C.P., C.I.C.

Stanford University, School of Medicine
...The current version of the HIV-1 Monitor test which uses RT-PCR may miss, or detect but be unable to quantitate subtype A plasma RNA. Modifications to PCR gene Read more »

DNA PCR missing some subtypes?

I have read on this forum and on various online articles that the Qualitative DNA PCR can miss... Read more »

autoimmune disease and HIV test

Dr Conway: I have Uveitis and use Prednisolone eye drops for last 5 years on and off. I have done 7... Read more »
Articles
New York State Department of Health AIDS Institute

Diagnostic, Monitoring and Resistance Laboratory Tests for HIV

...Assays 2. HIV-1 Confirmatory Antibody Assays 3. HIV-2 Antibody Screening B. Viral Identification Assays 1. DNA Polymerase Chain Reaction (DNA-PCR) 2. Plasma HIV RNA Assays III. Monitoring Tests A. Lymphocyte Analysis ... Read more »
Brown Medical School

Ask the Expert: A New Infection With an Undetectable Viral Load

...B viruses, not all viral load assays can detect virus in the presence of non-B subtypes (e.g., Roche Amplicor Version 1.0 or reverse transcriptase-polymerase chain reaction [RT-PCR] 1.0). Those that can detect it (branched DNA [b-DNA], Nuclisens,... Read more »
The Well Project

Understanding HIV-Related Lab Tests II: Viral Load, Resistance and Tropism

...Viral Load Test Viral load is the amount of HIV in your bloodstream. Viral loadcan be measured by two different lab tests: polymerase chain reaction (PCR) orbranched DNA (bDNA). It is best to stick with the same kind of test and notswitch... Read more »
The Well Project

Women and HIV Viral Load

...of HIV, the greater the risk of your immune system being damaged. Viral load can be measured by two different lab tests: a polymerase chain reaction (PCR) test or a branched DNA (bDNA) test. Both tests are accurate, but each has a different way... Read more »
Connect With Others

Broken Condom New HIV strain or What? WORRIED

Posted by ademalu, 1 Reply
...HIV+ and later changed she was joking she is HIV-. Is the Elisa (elecsys) for HIV 1 and 2 able to detect antibodies to all subtypes for HIV 1 and 2? Is the QL DNA PCR able detect genetic material for all subtypes for HIV 1. Since i cant not... Read more »

PCR and subtypes

Posted by Anonymous
...HIV-1 Proviral DNA PCR test. The kit used for the test is artus RealArt RG RT-PCR. Does anyone know if it covers all the major subtypes ..i.e A, B, C, D, E, F& G. I tried calling the concellor at th testing center and he did not give me... Read more »

PCR and subtypes

Posted by Anonymous
...HIV-1 Proviral DNA PCR test. The kit used for the test is artus RealArt RG RT-PCR. Does anyone know if it covers all the major subtypes ..i.e A, B, C, D, E, F& G. I tried calling the concellor at th testing center and he did not give me... Read more »

HIV DNA reliability

Posted by Anonymous, 1 Reply
...case in point. See below. "It has been reported that commercial qualitative HIV proviral DNA tests may not detect all genetic subtypes of HIV. One study reports that although100% of subtype B (n=16), G (n=9) and D (n=1)were detected, variable... Read more »