What Does An Oral Assay Test For

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Donor Screening Assays for Infectious Agents and HIV Diagnostic Assays

Antibody to Hepatitis B Surface Antigen (HBsAg Assay) Tradename(s) Format Sample Use Manufacturer Approval Date Auszyme Monoclonal EIA Serum / Plasma / Cadaveric Serum Donor Screen & Conf. Kit Abbott Laboratories... Read more »

Three New Assays Approved

On May 23, 2007, FDA approved the Procleix Ultrio Assay on the fully automated Procleix TIGRIS system manufactured by Gen-Probe Inc., of San Diego, California, and marketed by Chiron Corporation. This is a fully automated qualitative in vitro... Read more »

Can a Trofile Assay Be Used to Predict HIV Disease Progression?

There's nothing like hearing the results of studies directly from those who actually conducted the research. In this interview, you'll meet one of these impressive HIV researchers and read her explanation of a study she presented at the 49th... Read more »

Ask the Experts

HIV RNA Assay and PEP

Doctor Bob,About two weeks ago I went to a Bangkok Hospital to start PEP from what I thought was a high risk exposure to a female sex worker here in Thailand. It was protected vaginal intercourse (with exposure to blood) and unprotected oral. Under...

Response from Robert J. Frascino, M.D.

The Robert James Frascino AIDS Foundation

Hello,Yes, I absolutely agree: PEP is not warranted. (See below.)PCR technology has much more difficulty with false-positives than with false-negatives; however, please note PCR is not recommended for routine HIV screening for a variety of... Read more »

What is the deal with the VIRCO Assay(s)

I read that a group in Belgium, VIRCO, is developing something called the RT-antivirogram which provides phenotypic resistance testing of the nucleosideanalogue drugs and the non-nucleoside reverse transcriptase inhibitors. Is it only for nukes and...

Response from Mark Holodniy, M.D., F.A.C.P., C.I.C.

Stanford University, School of Medicine

The VIRCO antivirogram is an assay which takes the reverse transcriptase and protease enzymes from a patient's strain of HIV, and inserts them into the backbone of lab adapted strain of HIV. Initially used to look at the RT inhibitors, the... Read more »

APTIMA HIV-1 RNA QUALITATIVE ASSAY (APTIMA - HIV-1 RNA QUALITATIVE ASSAY, 2010)

Dear Dr. Bob,I thank you kindly and sincerely for your efforts, support and the invaluable knowledge you provide to the questioners on this forum. I have been reading this website extensively (over a month now) since my potential exposure date, and...

Response from Robert J. Frascino, M.D.

The Robert James Frascino AIDS Foundation

Hi Aptimistaken,Thanks for your kind comments! You are correct: the Aptima HIV-1 RNA Qualitative Assay is the only RNA PCR test approved by the FDA for HIV-diagnostic screening. You are also correct: the biggest problem with PCR assays is... Read more »

APTIMA HIV-1 RNA QUALITATIVE ASSAY (APTIMA - HIV-1 RNA QUALITATIVE ASSAY, 2010) Part 2

To the good Dr. Bob, I have submitted a donation on-line, and will submit a heftier holiday donation as well, but in the interim I was hoping you might field another inquiry from me regarding a post of mine a few weeks ago pertaining to the Aptima...

Response from Robert J. Frascino, M.D.

The Robert James Frascino AIDS Foundation

Hi Aptimistaken,You're going to qualify for an advanced degree in nucleic acid polymerase chain reaction laboratory analysis before long. Dude, it's time to turn off your inner scientific geek and come back to planet earth. I can't... Read more »

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antigen sandwich assays

Posted by Anonymous

Is this antigen sandwich assays part of the regular 3rd generation elisa test ?

could someone please explain the meaning of this ?
I know it sounds funny but I read that this test will pick up antibodies in 4 weeks.

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Abbott ARCHITECT HIV Ag/Ab Combo assay Accuracy

Posted by pupiz, 1 Reply

Hi, all. I had an exposure June 25 and ran across this particular 4th generation test that was approved by the FDA in 2010. I am not sure about the accuracy of it. I took an HIV test at 19 days and it was negative. I know that it is not conclusive,... Read more »

Testing At 12 Weeks

Posted by Anonymous, 1 Reply

I am wondering about the accuracy of the Elisa test at 12 weeks. I had oral sex with another male (both giving and receiving). He did not ejaculate in my mouth, but I'm not sure if there was any pre-cum. I do not recall having any symptoms 2-4... Read more »

HIV Testing

Posted by Anonymous, 1 Reply

Another question:
I went to my AIDS clinic for a test a while back. When I took the ELISA oral test and asked him about the time frame, he told me that after 25 days, the test is considered 99.9% accurate. Now I'm reading on here that the three... Read more »

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