What Are The Hiv Elisa Techniques

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Difficult (but important) question about different techniques to sequence the virus

Hi Doc, I understand Brian Conway's team (University of British Columbia) picked up an atypical case of HIV by sending a plasma sample for resistance test, even though the patient had negative ELISAs 12 and 14 months after exposure, negative...

Response from Mark Holodniy, M.D., F.A.C.P., C.I.C.

Stanford University, School of Medicine

This is too complex of a question to answer here. You are correct that PCR is used in the first step of the genotyping resistance assay followed by a sequencing reaction to determine the DNA sequence that uses different biochemical steps. PCR is not... Read more »

ELISA TEST

I was recently tested for Lyme Disease having been bitten by a deer tick. My understanding is that... Read more »

Response from Rick Sowadsky, M.P.H. »

Major Bob:Oraquick Tests vs. Normal Blood Elisas

Hey Doc, Ground Control again..I had a question about Oraquick Test vs. Elisas. The Oraquick which... Read more »

Response from Robert J. Frascino, M.D. »

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Articles

Researchers Developing Pasteurization Technique to Help HIV-Positive Women Breastfeed

VOA News on Thursday examined a project to help HIV-positive women in developing countries breastfeed their infants and reduce the risk of mother-to-child transmission. According to VOA News , some HIV-positive women often face a difficult... Read more »

New Techniques Aim to Sniff Out Hidden HIV

HIV infects cells of the immune system and reprograms them to spew out copies of the virus. But not all of these HIV-infected cells roam freely in the body; some HIV goes into hibernation instead, avoiding the attention of the immune system and of... Read more »

Researchers Develop Technique That Prevents HIV From Reproducing, Philadelphia Inquirer Reports

Researchers from the biotechnology company Virxsys and the University of Pennsylvania have developed a gene therapy technique that prevents HIV from reproducing, according to a study presented Wednesday at the 15th Conference on Retroviruses and... Read more »

Gene Technique That Alters CCR5-Producing Gene Increases Resistance to HIV in Mice, Study Finds

Altering the gene that produces CCR5 protein on the surface of immune cells using a harmless virus was found to significantly increase resistance to HIV in mice, according to a study published Sunday in the journal Nature Biotechnology , the Press... Read more »

Connect With Others

INFORMATION ABOUT ELISA TESTS

Posted by Anonymous

Jessica P. is a 32-year-old African American woman in good health. In November of 1999, she received a flu shot at her local clinic. Two weeks later, she developed fever and some upper respiratory tract complaints. She visited her primary care... Read more »

French Advice on ELISA

Posted by Anonymous, 1 Reply

Hello,

I have been following this message board for a while, and
I came across this information from the French website, in
their q/a forum.

The site is http://www.crips.asso.fr/

I can't give a link to the question, but it's question
no. 2488, and it's... Read more »

HIV ELISA Test Question

Posted by Anonymous, 2 Replies

One last question for all of you experts. I had an HIV test and it was negative. Does the ELISA test both HIV 1 & 2?? I have been in areas where HIV 2 is prevalent. However, while in those areas my risk was virtually non-existent. Just... Read more »

PCR/ELISA & "Hiding HIV"

Posted by Anonymous, 2 Replies

I tested negative on a standard ELISA test AND a DNA PCR test at almost 5 months post-exposure. The results were negative. Nevertheless, joint and node pain continues. I can't stop thinking that HIV is somehow hiding in my nodes and thus escaping... Read more »

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