What Are The Hiv Elisa Techniques

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Difficult (but important) question about different techniques to sequence the virus

Hi Doc, I understand Brian Conway's team (University of British Columbia) picked up an atypical case of HIV by sending a plasma sample for resistance test, even though the patient had negative ELISAs 12 and 14 months after exposure, negative...

Response from Mark Holodniy, M.D., F.A.C.P., C.I.C.

Stanford University, School of Medicine

This is too complex of a question to answer here. You are correct that PCR is used in the first step of the genotyping resistance assay followed by a sequencing reaction to determine the DNA sequence that uses different biochemical steps. PCR is not... Read more »

Are my Safe Sex techniques going too far?? Others should read too.

Hi Dr. Wohl, I'm sharing my story so hopefully your response can help educate myself and... Read more »

Response from David Wohl, M.D. »

"crazed HIV-positive phlebotomist with excellent aim and technique" guy again, scared as hell

Dear Dr Bob,Remember my little incident nearly two months ago at Las Vegas? I'm very thankful... Read more »

Response from Robert J. Frascino, M.D. »

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Articles

Researchers Developing Pasteurization Technique to Help HIV-Positive Women Breastfeed

VOA News on Thursday examined a project to help HIV-positive women in developing countries breastfeed their infants and reduce the risk of mother-to-child transmission. According to VOA News , some HIV-positive women often face a difficult... Read more »

U.S. Centers for Disease Control and Prevention

Scientists Develop Technique That Makes Some Immune Cells Resist HIV

Researchers have come up with a technique that makes certain types of immune cells resistant to HIV infection. The researchers believe that this could lead to a new approach to removing HIV from the body. According to James Stivers of Johns Hopkins... Read more »

New Techniques Aim to Sniff Out Hidden HIV

HIV infects cells of the immune system and reprograms them to spew out copies of the virus. But not all of these HIV-infected cells roam freely in the body; some HIV goes into hibernation instead, avoiding the attention of the immune system and of... Read more »

Researchers Develop Technique That Prevents HIV From Reproducing, Philadelphia Inquirer Reports

Researchers from the biotechnology company Virxsys and the University of Pennsylvania have developed a gene therapy technique that prevents HIV from reproducing, according to a study presented Wednesday at the 15th Conference on Retroviruses and... Read more »

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INFORMATION ABOUT ELISA TESTS

Posted by Anonymous

Jessica P. is a 32-year-old African American woman in good health. In November of 1999, she received a flu shot at her local clinic. Two weeks later, she developed fever and some upper respiratory tract complaints. She visited her primary care... Read more »

French Advice on ELISA

Posted by Anonymous, 1 Reply

Hello,

I have been following this message board for a while, and
I came across this information from the French website, in
their q/a forum.

The site is http://www.crips.asso.fr/

I can't give a link to the question, but it's question
no. 2488, and it's... Read more »

HIV ELISA Test Question

Posted by Anonymous, 2 Replies

One last question for all of you experts. I had an HIV test and it was negative. Does the ELISA test both HIV 1 & 2?? I have been in areas where HIV 2 is prevalent. However, while in those areas my risk was virtually non-existent. Just... Read more »

PCR/ELISA & "Hiding HIV"

Posted by Anonymous, 2 Replies

I tested negative on a standard ELISA test AND a DNA PCR test at almost 5 months post-exposure. The results were negative. Nevertheless, joint and node pain continues. I can't stop thinking that HIV is somehow hiding in my nodes and thus escaping... Read more »

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