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Viral Load Assay

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Ask the Experts

Viral Load Assay Question

Hello Dr. Holodiny,I notice on my lab report for my viral load under the Actual Kit copies/ml that it states <50 and then on another line is says HIV-1 RNA Not Detected. I know that 50 is the lower limit for detection for the LMDS-ROCHE...
Mark Holodniy, M.D., F.A.C.P., C.I.C.

Response from Mark Holodniy, M.D., F.A.C.P., C.I.C.

Stanford University, School of Medicine
...The assay can detect viral load below 50 but not reliably. Because of that, the lower limit of viral load detection is pegged at 50 copies, which is where the Read more »

down side of viral load assays

most labs are not always fool proof what if any are the misinterpretations of viral load readings... Read more »

increase in viral load

I have been positive for 4 years now and I am not on any meds. My viral load has always been in... Read more »
Articles
Test Positive Aware Network

Martell Randolph

...testing. The standard method of diagnostic testing used for managing HIV includes regular viral load and CD4 measurements. The viral load assay, or test, used to measure HIV-2, however, is different from the one that is used to detect HIV-1, and... Read more »
Gay Men's Health Crisis

Resistance Assay Update

...ritonavir to some extent. No person with resistance to both attained viral loads below 500, the limit of detection with the viral load assay in use. (Resistance determinations were made by Virco.) But the patients whose tests showed sensitivity... Read more »
New York State Department of Health AIDS Institute

Diagnostic, Monitoring and Resistance Laboratory Tests for HIV

...Polymerase Chain Reaction (DNA-PCR) 2. Plasma HIV RNA Assays III. Monitoring Tests A. Lymphocyte Analysis B. Viral Load Assays 1. Roche Amplicor HIV-1 Monitor and Roche Amplicor HIV-1 Monitor Ultrasensitive (RT-PCR) 2.... Read more »
Gay Men's Health Crisis

The Search for More Intelligent Therapy

...breakthrough, and for that, Geneva could offer only some clues. Statistical Magic Obscures the True Response Rate The introduction of viral load assays in the last two years has brought a revolution in monitoring the progress of HIV infection... Read more »
Connect With Others

some one with more knowledge please explain this

Posted by chrisbadmushroom, 1 Reply
Does this mean they cured HIV in these patents? http://www.ncbi.nlm.nih.gov/pubmed/19031451 Immunotherapy of HIV-infected patients with Gc protein-derived macrophage activating factor (GcMAF). Yamamoto N, Ushijima N, Koga Y. Division of... Read more »

GI Bleeding

Posted by Anonymous, 2 Replies
At 2 weeks after i began to have symptoms sore throat, cough, lung infection, light headed. 2 months after a possible infection i began to have black stool. I then got Bronchitis @ 2 months. Since then i have been having burning in mouth, a single... Read more »

Do I or Do I Don't

Posted by LostSould, 1 Reply
...asked for another test. I was tested June 14, 2010 and today my doctor said the results was the same and I had no detectable viral load. I am so scared. I was told to come back in August then November for further testing. I am going crazy. I am... Read more »

sjb1 explain this from dr.gallant

Posted by mental_case
...PCR tests. Can you describe these in more detail? The quantitative HIV RNA is what is typically referred to as the "viral load" assay. It measures the amount of HIV virus in plasma. The qualitative test is an HIV DNA PCR, which simply... Read more »