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Proviral Dna Test As Hiv Test

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HIV-1 Proviral DNA by PCR test????

Posted by sadman, 2 Replies
Hello everyone thanks for putting up with me. Its been 8 weeks since my risk and I'm more concerned then ever. I really do seem to have every symptom of ARS despite assurances risks were very slight to none existent.

That said, does anyone know... Read more »

Proviral DNA PCR Test--Please answer

Posted by Anonymous, 1 Reply
I had an oral sex encounter in a gay bathhouse about 6 weeks ago. We both gave each other blow jobs without a condom. I came in his mouth...he did not cum in mine.

I have been tested for all STDs (syph, chlamydia, gonnoreah, hep B, herpes, etc),... Read more »


Posted by Anonymous, 3 Replies
Does anyone recommend the DNA PCR test i.e. the test that looks for HIV directly rather than HIV antibodies. Does anyone have any experience of this test and if so did it put your mind at ease. I did read that there's a high degree of false... Read more »

how accurate is hiv dna test

Posted by gpboy, 1 Reply
i have given hiv dna pcr test on 9th day after probable exposure.

i am waiting for the results in next two days. can any one say how much i can trust this test results? Read more »

HIV DNA RT by PCR test and PEP medications

Posted by johnnyyop2011, 1 Reply
On June 16, I discovered I was exposed several times to HIV from March 15 to June 10.

I tested negative via an antibody tests both in April and on June 17.

On June 17, I was immediately put on Post Exposure Prophylaxis medications (Kaletra 200-50 mg... Read more »
Ask the Experts

ProviralDNA PCR test and hiv mutation

Thanks for answering my questions.This is a follow up question to the answer.Doctor as you have said that as the hiv mutates its DNA structure changes then will the Same DNA PCR test able to detect the virus?Also after the asymptomatic stage of...
Mark Holodniy, M.D., F.A.C.P., C.I.C.

Response from Mark Holodniy, M.D., F.A.C.P., C.I.C.

Stanford University, School of Medicine
The DNA test will detect virus, as the area of the HIV viral sequence that is used for the test is in a highly conserved area, which is not as prone to mutations. I am not sure what you mean by third stage. Symptoms in general are much less likely,... Read more »

Does Proviral DNA PCR test detect antigens?

I have following doubts,1)Does proviral DNA PCR test detect antigens?Is it any one antigen or all antigens?2)Is it possible that over a period of infection hiv mutates so that the antigens change and so that DNA PCR cannot detect changed antigen or...
Mark Holodniy, M.D., F.A.C.P., C.I.C.

Response from Mark Holodniy, M.D., F.A.C.P., C.I.C.

Stanford University, School of Medicine
DNA PCR detects DNA and not antigens, which are proteins. The DNA sequence can mutate or change its composition, which can then change the structure of the protein or antigen. DNA PCR also does not detect antibodies. I have not heard of HIV DNA PCR... Read more »

HIV-1 proviral DNA by PCR

This is the description of a $260 test on Private MD Labs's website. Is this a good early detection test? The company says its conclusive at 28 days. So I'm asking did I waste my $260 by taking this test at 16 days post incident? Would...
Robert J. Frascino, M.D.

Response from Robert J. Frascino, M.D.

The Robert James Frascino AIDS Foundation
Hi,Qualitative DNA PCR is used to detect cell-associated proviral DNA. The test is not considered to be sufficiently accurate for making an HIV diagnosis without confirmation and is not FDA approved for this use. However, it can be helpful to sort... Read more »

Only DNA proviral was positive for HIV 2

Im from Portugal. I work in National Health System and I've heard about a black patient from Africa with pancytopenia and leishamania.CD4 was very low(<200). Antibody to HIV comes negative, as well WB. Only DNA proviral was positive for HIV...
David Wohl, M.D.

Response from David Wohl, M.D.

University of North Carolina - Chapel Hill
I am not sure of the specific here but could be the patient had HIV-2 and was tested for only HIV-1. In most places (like the US) the HIV antibody test looks for both viruses but could be in others just HIV-1 is looked for. I do not this his low... Read more »
U.S. Centers for Disease Control and Prevention

Reasons for Not HIV Testing, Testing Intentions and Potential Use of an Over-the-Counter Rapid HIV Test in an Internet Sample of Men Who Have Sex With Men Who Have Never Tested for HIV

The correlates of the main reasons for not HIV testing, HIV testing intentions, and the potential use of an over-the-counter rapid HIV test (OTCRT) among men who have sex with men who have never tested for HIV (NTMSM) are unknown, the authors wrote.... Read more »
U.S. Centers for Disease Control and Prevention

DNA Testing Method Can Identify Twice as Many Throat and Rectal Gonorrhea and Chlamydia Infections as Traditional Bacterial Culture

A new study shows that a more efficient STD screening method -- called a nucleic acid amplification test (NAAT) -- can detect at least twice as many oropharyngeal (throat) and rectal gonorrhea and chlamydia infections as a bacterial culture test,... Read more »

How Accurate Are Rapid HIV Tests? Study Finds Blood Tests Slightly More Accurate Than Oral Tests

Rapid HIV tests perform slightly worse when testing oral samples in comparison to blood, according to a new study published in The Lancet Infectious Diseases on Jan. 24. Researchers from Canada, led by Nitika Pant Pai, M.D., of McGill... Read more »
New York State Department of Health AIDS Institute

Diagnostic, Monitoring and Resistance Laboratory Tests for HIV

Table of Contents I. Introduction II. Diagnostic Tests A. Antibody Tests 1. HIV-1 Antibody Screening Assays 2. HIV-1 Confirmatory Antibody Assays 3. HIV-2 Antibody Screening B. Viral Identification Assays 1. DNA Polymerase... Read more »