Does Pcr Dna Detect All Subtypes

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Ask the Experts

HIV DNA PCR and Subtypes

Dear Doctor Holodniy:I understand that the RT-PCR viral load test may miss subtype A. What about the DNA PCR test? Will it detect proviral dna associated with all HIV-1 subtypes? Thank you for your time....

Response from Mark Holodniy, M.D., F.A.C.P., C.I.C.

Stanford University, School of Medicine

The current version of the HIV-1 Monitor test which uses RT-PCR may miss, or detect but be unable to quantitate subtype A plasma RNA. Modifications to PCR gene primers have been made and are currently being tested which allow for this detection. The... Read more »

DNA PCR missing some subtypes?

I have read on this forum and on various online articles that the Qualitative DNA PCR can miss... Read more »

Response from Mark Holodniy, M.D., F.A.C.P., C.I.C. »

DNA PCR - Concern about Rare Subtypes

Hi, Richard. I had a DNA PCR done at my doctor's office in 2008. Would this have detected all... Read more »

Response from Richard Cordova III »

Go to Ask the Experts »

Articles

U.S. Centers for Disease Control and Prevention

Type of AIDS Infection Key to Death Risk -- Study

The clade, or subtype, of HIV infection was a better predictor of rapid AIDS mortality than viral load in a Ugandan study recently presented at the 13th Conference on Retroviruses and Opportunistic Infections in Denver. Conducted by US and Ugandan... Read more »

HPV Test More Accurate Than Pap Test at Detecting Cervical Cancer, NEJM Studies Say

Human papillomavirus tests are more accurate than Pap tests in the detection of cervical cancer and precancerous changes in the cervix, according to two studies published on Thursday in the New England Journal of Medicine , the Wall Street Journal... Read more »

Portable Device Detects HIV With Push of a Button

After approximately 10 years of work, researchers at the California Institute of Technology (Caltech) have built an inexpensive, portable, easy-to-use device to quickly diagnose HIV/AIDS and other diseases. Axel Scherer and George Maltezos began... Read more »

Many STIs Not Detected in U.S. Because of Lack of Testing Among MSM, Health Officials Say

Many cases of sexually transmitted infections are not being detected in the U.S. because of a lack of testing among men who have sex with men, health officials said Wednesday at a scientific meeting in Chicago, the New York Times reports.... Read more »

Connect With Others

PCR not detecting all strains of HIV?

Posted by Anonymous, 1 Reply

As many worried persons, I took a PCR-RNA 4 weeks after my infection risk, to amend my fears. It came back negative. Although I should relax now, I'm still concerned, because I have read that all commercially available PCR-kits are just looking for... Read more »

PCR and subtypes

Posted by Anonymous

Hi,

Can anyone tell me if they have heard of HIV-1 Proviral DNA PCR test. The kit used for the test is artus RealArt RG RT-PCR.
Does anyone know if it covers all the major subtypes ..i.e A, B, C, D, E, F& G. I tried calling the concellor at th... Read more »

PCR and subtypes

Posted by Anonymous

Home Access & PCR DNA Information

Posted by Anonymous

Excellent post about Home Access efficacy, sensitivity, and the subtypes it now tests for is available in Dr. Holodniy's Viral Load forum on this site. Also please note yet another posting regarding PCR DNA. Both are dated July 29, so you... Read more »

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