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Does Pcr Dna Detect All Subtypes

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Ask the Experts

DNA PCR missing some subtypes?

I have read on this forum and on various online articles that the Qualitative DNA PCR can miss detecting some subtypes of HIV, especially non-B subtypes. This is mind, how can the Qualitative DNA PCR be considered a good test for HIV if the test...
Mark Holodniy, M.D., F.A.C.P., C.I.C.

Response from Mark Holodniy, M.D., F.A.C.P., C.I.C.

Stanford University, School of Medicine
...The overwhelming majority of infected people in the US have subtype B. Certain DNA PCR tests can miss some of the subtypes. Others might pick them up. Several current antibody tests can pick them up. Read more »

HIV DNA PCR and Subtypes

Dear Doctor Holodniy:I understand that the RT-PCR viral load test may miss subtype A. What about... Read more »

Can HIV sub-type O be detected by PCR?

Dear Dr, Can the HIV sub-type O be tested by HIV PCR after 10 months from exposure? I'm really feel... Read more »
Articles
New York State Department of Health AIDS Institute

Diagnostic, Monitoring and Resistance Laboratory Tests for HIV

...Assays 2. HIV-1 Confirmatory Antibody Assays 3. HIV-2 Antibody Screening B. Viral Identification Assays 1. DNA Polymerase Chain Reaction (DNA-PCR) 2. Plasma HIV RNA Assays III. Monitoring Tests A. Lymphocyte Analysis ... Read more »
Brown Medical School

Ask the Expert: A New Infection With an Undetectable Viral Load

...B viruses, not all viral load assays can detect virus in the presence of non-B subtypes (e.g., Roche Amplicor Version 1.0 or reverse transcriptase-polymerase chain reaction [RT-PCR] 1.0). Those that can detect it (branched DNA [b-DNA], Nuclisens,... Read more »
U.S. Centers for Disease Control and Prevention

Updated Interim Recommendations -- HIV-Infected Adults and Adolescents: Considerations for Clinicians Regarding 2009 H1N1 Influenza

...influenza diagnostic tests (RIDTs) and direct immunofluorescence assays (DFAs) are lower than real-time reverse transcriptase polymerase chain reaction (rRT-PCR) tests and viral culture. A negative RIDT or DFA result does not rule out influenza... Read more »
AIDS InfoNet

Viral Load Tests

...Load? The viral load test measures the amount of HIV virus in your blood. There are different techniques for doing this: The PCR ( p olymerase c hain r eaction) method uses an enzyme to multiply the HIV in the blood sample. Then a chemical... Read more »
Connect With Others

PCR and subtypes

Posted by Anonymous
...HIV-1 Proviral DNA PCR test. The kit used for the test is artus RealArt RG RT-PCR. Does anyone know if it covers all the major subtypes ..i.e A, B, C, D, E, F& G. I tried calling the concellor at th testing center and he did not give me... Read more »

PCR and subtypes

Posted by Anonymous
...HIV-1 Proviral DNA PCR test. The kit used for the test is artus RealArt RG RT-PCR. Does anyone know if it covers all the major subtypes ..i.e A, B, C, D, E, F& G. I tried calling the concellor at th testing center and he did not give me... Read more »

Broken Condom New HIV strain or What? WORRIED

Posted by ademalu, 1 Reply
...HIV+ and later changed she was joking she is HIV-. Is the Elisa (elecsys) for HIV 1 and 2 able to detect antibodies to all subtypes for HIV 1 and 2? Is the QL DNA PCR able detect genetic material for all subtypes for HIV 1. Since i cant not... Read more »

What do yo mean?!?

Posted by Anonymous, 3 Replies
...detect Sybtype B more than other strains thinks. Whats wrong with your goverment?!? I can say this, sweden have generally all subtypes in the country thats exists and that why we are using HIV 1/2 tests that also detects o strain even if this... Read more »