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Does 3rd Generation Elisa Miss Any Subtypes

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Difference between 1st generation and 3rd

Posted by Anonymous, 1 Reply
I found this on THIS website:

About Home Access and first generation testing have confidence in it-

"We do use the first generation ELISA, which uses viral lyzates as antigen. The difference with the third generation ELISA is that the latter uses... Read more »

3rd Generation Elisa test

Posted by Protus, 2 Replies
How can I be sure that the test I had done was a 3rd generation elisa?

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Elisa 3rd. generation

Posted by Stormbringer, 1 Reply
How much can I trust in my result after 46 days? Read more »

3rd Generation ELISA

Posted by Anonymous, 1 Reply
I heard that 3rd generation ELISA's are more sensitive but I'm not sure what I was tested with. How can I find out? If I was tested in 2003 would they have used the 3rd generation tests? Please help. Read more »

3rd or 4th generation elisas?

Posted by Anonymous, 1 Reply
I was wondering if any county health dept. around the country would be using elisas first generation. Is everyone using 3rd or 4th generation? Read more »
Ask the Experts

3rd generation Elisa??????

DEAR RYAN,PLEASE ANSWER-I have been testing for HIV for exactly 5 months. My doctor has been using the ELISA and PCRS all have come back negative.I thought the Elisas would be 3rd or 4th Generation-THEY ARE NOT.THEY ARE ONLY 1ST GENERATION-MY DOCTOR...
Ryan M. Kull, C.S.W

Response from Ryan M. Kull, C.S.W

Columbia University's Health & Related Services
It is not necessary to perform a third generation ELISA at this point. A first generation ELISA performed repeatedly over five months following exposure is sufficient. The majority of people will still develop a detectable level of antibodies, as... Read more »

ELISA Test Generation. Please reply...its my 3rd post. HELP!

Dr Bob,I have posted a few times previously and hope you can spare some time to reply to my question.I am from Malaysia and had a risky exposure. I have been testing many times (About 5-6 times) for the last 5 months. My latest test was at the 22...
Robert J. Frascino, M.D.

Response from Robert J. Frascino, M.D.

The Robert James Frascino AIDS Foundation
Hi,1.Yes.2.Yes, but I very strongly doubt what you are palpating is related to HIV-associated lymphadenopathy.3.Yes. No.4.No. Antibiotics, such as penicillin used to treat syphilis, will not delay HIV-antibody development.My advice:1.Stop poking... Read more »

3rd generation tests

WOnder if you could please reply :1] When were 3rd generation ELISA tests introduced ?2] When are 4th generation tests expected to be introduced ?...
Mark Holodniy, M.D., F.A.C.P., C.I.C.

Response from Mark Holodniy, M.D., F.A.C.P., C.I.C.

Stanford University, School of Medicine
3rd generation tests were introduced a few years ago. Fourth generation tests, are considered by many to already be on the market. They include both HIV antibody and p24 antigen tests in one test.... Read more »

3rd and 4th generation test

You previously mentioned that the different generations of hiv tests differ in types of hiv-1 they detect.Can 3rd or 4th gen. test pick up all the strains we know about(A-h,and O )? What strains would a first generation test detect? Would it matter...
Mark Holodniy, M.D., F.A.C.P., C.I.C.

Response from Mark Holodniy, M.D., F.A.C.P., C.I.C.

Stanford University, School of Medicine
The generational definition is partially historic. Most testing laboratories are using current generation antibody assays. Some viral load assays have difficulty with some subtypes of HIV-1. But again, these are not approved for diagnosis.MH... Read more »

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