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Accuracy Of Pooled NAT Testing For Hiv

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Get the Facts
San Francisco AIDS Foundation

HIV Testing

...infection stage. Many feel that identifying and making interventions during this period will greatly reduce infection rates. A cost-effective and efficient way to combine standard ELISA tests with viral load tests is called a "pooled PCR". Viral load tests are expensive to do individuallys. Instead of performing viral load tests on individual blood samples, they have found a way to combine or "pool" samples and test multiple people's...
Ask the Experts

HIV screening for donating blood (BLOOD DONATION, FALSE POSITIVE TEST 2009)

Dr. Bob, My sister recently tried to donate blood and was denied. She recieved a letter stating that she was HIV positive. How accurate are the tests that the Red Cross performs? She is getting an HIV test but I would like to know what the...
Robert J. Frascino, M.D.

Response from Robert J. Frascino, M.D.

The Robert James Frascino AIDS Foundation
...tested as a batch. If the batch tests positive, then each of the 20 donor samples is tested individually by our usual serological testing methods. See, I told you this was a bit confusing. As for your second question, my occupational exposure was... Read more »

Transmission from menstrual period in a pool!

I previously asked this question. Tested at 3, 6 and 9 months (negative) but have had persisten symptoms (sore throat); was in a pool with my children, did not know had my period; they started getting symptoms (sore throat)this has continued for all...
Robert J. Frascino, M.D.

Response from Robert J. Frascino, M.D.

The Robert James Frascino AIDS Foundation
...Hello,HIV cannot be transmitted via swimming pools, whether an HIVer had her period while doing laps or was profusely bleeding from a slashed artery while swimming. No risk. None. Read more »

test2

Dr. Bob,Although it might sound strange, I enjoy skinny dipping in the Lake. I usually do it where there are no people around so I don't freak anyone out...plus I am really not that well hung!!. Is there any risk of catching hiv or hepatitis in...
Judith Feinberg, M.D.

Response from Judith Feinberg, M.D.

University of Cincinnati, College of Medicine
asdfasdf Read more »

Confusion Over RNA/NAAT Test

Dr. Bob,I donate every year and will continue to do so but hope you will answer this question. Had unprotected oral (giving/receiving with no ejaculation) and protected receptive anal; saw condom afterwards and not broken. Probably only need to...
Robert J. Frascino, M.D.

Response from Robert J. Frascino, M.D.

The Robert James Frascino AIDS Foundation
...testing they offer and whether or not it is reliable. also, is the chance for a false positive still high as i believe it is a pooled nat test? whether or not you answer this, thanks for all your help and support, you've gotten me through a scare... Read more »
Articles
The Well Project

Myths About HIV

...needle that has been used by someone who is HIV positive. The myth: "HIV can be spread through tears, sweat, mosquitoes, pools, or casual contact." The reality: HIV can only be transmitted through infected blood, semen, vaginal... Read more »
AIDS InfoNet

Stopping the Spread of HIV

...into your system. You can't get it from mosquito bites, coughing or sneezing, sharing household items, or swimming in the same pool as someone with HIV. Some people talk about "shared body fluids" being risky for HIV, but no documented cases of... Read more »
Henry J. Kaiser Family Foundation

Baltimore Launches HIV Nucleic Acid Testing Program

...developed in 1999, is used to test units of donated blood for HIV. NAT can detect minute amounts of viral genetic material in pooled plasma samples by amplifying gene fragments of the virus. If a pool tests positive for HIV, the individual... Read more »
Henry J. Kaiser Family Foundation

Botswana's HIV Prevalence Reducing Country's Blood Donor Pool, Red Cross Official Says

... Botswana's high HIV prevalence has reduced the country's pool of blood donors while also increasing the need for blood services in the country, Nomsa Mbere, president of the Botswana Red Read more »
Connect With Others

The RNA test.

Posted by TrueOptimist, 1 Reply
I have took the I believe it is called an "RNA" test because no antibodies have been found in my body so this test will look for the virus itself in my blood.I just want to know is this very accurate? Read more »

NAT testing

Posted by Anonymous, 2 Replies
...Does anyone know anything about Nucleic Acid Testing (NAT) I was told that blood banks use it to seek the virus in donated Blood. How effective is it? I have had numberous negitive Read more »

hiv viral load

Posted by Anonymous, 1 Reply
...Pilcher and his colleagues, who run the North Carolina viral load testing program, explain that all HIV negative blood samples are pooled into batches of 90 and tested. If a batch tests positive, it is examined sample by sample until the positive... Read more »

That old debate...

Posted by Anonymous, 3 Replies
...HIV and HCV could have slipped under the radar in the tests the Red Cross do on blood? I understand they do something called pooled NAT tests. Could pooling the blood with 19 other people's ever cause the virus/es to be so diluted that they... Read more »