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Accuracy Of ELISA And Western Blot

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Western Blot Accuracy

Posted by brainfried, 3 Replies
I have read tons of articles on Elisa testing and all the various rapid tests, but I can't find any information at all on the Western Blot test. From my understanding, the Elisa has a high sensitivity which means you will get some(rarely) false... Read more »

ELISA+WESTERN BLOT?

Posted by Anonymous, 1 Reply
I have a question for you in the U.S, Do you always use both Elisa and Western Blot(Even if Elisa is Negative) or do you only use Western Blot when the ELISA is positive?

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elisa positive and western blot negative

Posted by Anonymous, 1 Reply
Is there still a need to go for further test if 2 elisa positive and then negative western blot test. I am not in the risk group. Only some 9 months back I did unprotective oral but safe penetrating with al low risk girl.
I did 2 rapid also but all... Read more »

oral swab test - elisa + western blot

Posted by Anonymous, 1 Reply
Does anyone know about this oral swab test they give at free clinics? Are they as sensitive as a real blood test? The test consists of an oral thing you hold against your gums for awhile, and then they do 2 elisa screens on it, followed by a western... Read more »

Positive Elisa Negaive Western Blot

Posted by Thnkful, 1 Reply
I am just posting to let others who may be going though awaiting results that your mind can make you think the worst. I recently got a call from my doctor after a routine physical that I had tested positive on my Elisa and negative on the western... Read more »
Ask the Experts

Pregnant / positive elisa but negative western blot (POSITIVE ELISA, NEGATIVE WESTERN BLOT, PREGNANT, 2011)

My OBGYN advised me that my hiv results came back as a "repeatedly reactive" elisa but "negative" western blot. I am currently 10 weeks pregnant. I tested twice (5/2010 & 10/2010) for hiv while pregnant with my 8 month old...
Robert J. Frascino, M.D.

Response from Robert J. Frascino, M.D.

The Robert James Frascino AIDS Foundation
Hello.A repeatedly reactive (positive) ELISA and negative confirmatory Western Blot (WB) is considered a negative HIV test result, because the WB test is the more specific testing assay. Pregnancy can induce the production of nonspecific antibodies,... Read more »

Western Blot 'Indeterminate', ELISA 'Negative' (ELISA NEGATIVE, WESTERN BLOT INDETERMINATE)

Hi, I'm a male late 30s. I know you've touched on this issue before, but I was hoping to get more specifics on my somewhat unique quandry.My girlfiend insisted that I do a Western Blot _after_ my ELISA test. The ELISA for HIV 1/2 came...
Robert J. Frascino, M.D.

Response from Robert J. Frascino, M.D.

The Robert James Frascino AIDS Foundation
Hi,How can I explain specifics on the results of a test that should never have been ordered? Your ELISA HIV-1/2 is negative, so you are HIV negative. Period. End of story."Uninterpretable results" are exactly that... Read more »

Pregnant, + ELISA, indeterminate western blot (PREGNANCY AND INDETERMINATE WESTERN BLOT TEST, 2010)

I saw some threads about this subject - my OB informed me of a positive ELISA test and indeterminate western blot for HIV in my pregnancy bloodwork. He has now drawn more blood to send off for a PCR test. From research I've done, it is really...
Robert J. Frascino, M.D.

Response from Robert J. Frascino, M.D.

The Robert James Frascino AIDS Foundation
Hello,While not "common," I certainly would not consider indeterminate HIV-antibody testing during pregnancy to be "really rare" either! Check out the archives of this forum. There you will find a chapter devoted to... Read more »

Dr Robert is an Hiv WesternBlot (ELISA versus WESTERN BLOT tests for diagnostic screening)

Dr Robert is an Hiv Western Blot alot better then reg HIv 1/2 Elisa. Yeah I had My Dr run them both tests and they are Negative. Thanks' ...
Robert J. Frascino, M.D.

Response from Robert J. Frascino, M.D.

The Robert James Frascino AIDS Foundation
Hello,Western Blot tests should only be run to confirm a repeatedly reactive (positive) ELISA (or EIA or rapid test). It should not be used as a diagnostic tests without a positive initial screening test, due to the possibility of false-positive or... Read more »
Articles
San Francisco AIDS Foundation

Prueba del VIH

¿Por qué debería hacerse la prueba del VIH? ¿Qué es la prueba de anticuerpos contra el VIH? Elisa Ensayo Western Blot (WB) Ensayo de inmunofluorescencia indirecta (IFA) Período ventana ... Read more »
Los Foros "Pregunte al Experto" de The Body

Preguntas Frecuentes Acerca de la Prueba del VIH

¿Qué tan buena es la prueba PCR cualitativa para detectar la infección por VIH? En el resultado de mi prueba ELISA dice que soy portadora de VIH-1 ¿Qué diferencia hay entre VIH-1 y VIH-2? Después de meses de pruebas negativas, mi prueba... Read more »
Rick Sowadsky, M.S.P.H.

HIV Antibody Tests -- Now You Have Several Choices

Several years ago, the only type of antibody test available was a blood test done through your doctor's office. Today, as technology has improved, there are now different types of antibody tests available. Antibody tests can now be done on blood,... Read more »
San Francisco AIDS Foundation

HIV Testing

Why Should You Get Tested for HIV? What Are HIV Antibody Tests? ELISA Western Blot (WB) Assay Indirect Immunofluorescence Assay (IFA) Window Period Interpretation of Test Results Accuracy... Read more »