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RNA PCR In Absence Of Antibodies

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Ask the Experts

Adopting Child with initial positive then negative tests

We are considering adopting a child with the following results: 02/04 born;04/04 negative PCR HIV;07/04 negative PCR HIV;09/04 positive EIA to HIV;12/04 negative EIA to HIV;08/05 hiv monitoring stopped. What does it all mean?
Katherine Luzuriaga, M.D.

Response from Katherine Luzuriaga, M.D.

University of Massachusetts, Medical School
...An EIA (enzyme immunoassay) detects antibodies (an immune response) to HIV while the PCR (polymerase chain reaction) is a test that detects HIV genetic material (DNA or RNA). All HIV infected women have HIV antibodies and are EIA positive. During Read more »

PCR RNA / Antibodies

Hi doc.Have read that a person can have PCR RNA test result < 50 copies / ml. and still be HIV... Read more »

Viral Load Not Reliable after period of time???

My gynecologists tells me that the PCR RNA is not reliable. Now after having a negative antibody... Read more »
New York State Department of Health AIDS Institute

Diagnostic, Monitoring and Resistance Laboratory Tests for HIV

...Assays 2. HIV-1 Confirmatory Antibody Assays 3. HIV-2 Antibody Screening B. Viral Identification Assays 1. DNA Polymerase Chain Reaction (DNA-PCR) 2. Plasma HIV RNA Assays III. Monitoring Tests A. Lymphocyte Analysis ... Read more »
U.S. National Institute of Allergy and Infectious Diseases

The Evidence That HIV Causes AIDS

...communication). HIV can be detected in virtually everyone with AIDS. Recently developed sensitive testing methods, including the polymerase chain reaction (PCR) and improved culture techniques, have enabled researchers to find HIV in patients... Read more »
U.S. Centers for Disease Control and Prevention

Guidelines for Prevention and Treatment of Opportunistic Infections in HIV-Infected Adults and Adolescents

...antiviral therapy. Serial quantitative HCV RNA testing should be limited to persons receiving HCV treatment. Diagnostic assays using reverse transcriptase-polymerase chain reaction (RT-PCR) or transcription-mediated amplification (TMA) have been... Read more »
The Body PRO

Can HIV be Eradicated?: Report on Dr. David Ho's Presentation

...blunting if not lack of production of gp120 and p24 antibodies as compared to untreated patients. In 7 cases there was also an absence of fresh unstimulated cytotoxic T lymphocyte (CTL) response in their peripheral blood mononuclear cells. Ho... Read more »
Connect With Others

Accuracy of PCR

Posted by Anonymous, 2 Replies a 6 month ELISA (and certainly a 3 month ELISA). A thought occurred to it possible that one could develop enough antibodies to make the PCR undetectable at 7.5 weeks? The Quest test says it measures down to 10 copies. Do antibodies... Read more »


Posted by Anonymous
... ?We observed that clade A strains [of HIV] were not detected by RT-PCR [Reverse Transcriptase-Polymerase Chain Reaction] and that clade G-strains were not detected by NASBA [Nucleic... Read more »

elisa vs. pcr

Posted by Anonymous
...Whether extended follow-up is indicated in other circumstances (e.g., exposure to a source coinfected with HIV and HCV in the absence of HCV seroconversion or for exposed persons with a medical history suggesting an impaired ability to develop an... Read more »


Posted by Anonymous
...(sometimes even months) after the infection is acquired. On the other hand, the quantitative plasma HIV-1 RNA level (viral load) by polymerase chain reaction (PCR), which is 95 to 98 percent sensitive for HIV,8 becomes positive within 11 days of... Read more »