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Qualitative Dna By PCR At 4 Months

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Get the Facts
AIDS InfoNet

Viral Load Tests

...have found any HIV in the blood. The test can be used for diagnosis , because it can detect a viral load a few days after HIV infection. This is better than the standard HIV (antibody) test, which can be "negative" for 2 to 6 months after HIV infection. (See Fact Sheet 102 for more information on HIV antibody testing.) For prognosis , viral load can help predict how long someone will stay healthy. The higher the viral load, the faster...
Ask the Experts

Final Word on the DNA PCR

Doc,With a person who has a severe autoimmune disease (lupus) and has all the symptoms of HIV after a high risk contact, but HIV Antibody test is negative after 4 months 2 weeks, doctor suggested qualitiative DNA test (which I read to be the DNA...
Robert J. Frascino, M.D.

Response from Robert J. Frascino, M.D.

The Robert James Frascino AIDS Foundation
...Hello,I would agree with the use of a qualitative DNA PCR in your situation, even though the test is not FDA approved for routine HIV screening. I find it quite helpful in cases Read more »

Where to get tested using qualitative DNA PCR

Hi, Dr. Holodniy.I am interested in getting a qualitative DNA PCR test performed at 4 weeks from exposure (August 17). My family physician doesn't think I need the test - he says I should wait until 3 months and get the antibody (ELISA) test. The...
Mark Holodniy, M.D., F.A.C.P., C.I.C.

Response from Mark Holodniy, M.D., F.A.C.P., C.I.C.

Stanford University, School of Medicine
There are many national commercial labs that offer this test. The AIDS clinic should be able to tell you.MH Read more »


Dear Dr. Holodniy:I have a question on "Qualitative DNA PCR", but beforeI ask that I thought I would attempt to summarize yourprevious comments on the topic of PCR, so as toreduce repetitive questions and leave you more timeto answer the new ones...
Mark Holodniy, M.D., F.A.C.P., C.I.C.

Response from Mark Holodniy, M.D., F.A.C.P., C.I.C.

Stanford University, School of Medicine
...Great job on 1-10!!! There are some semantic arguments here regarding quantitation. In some respects all PCR tests are quantitative, in that each cycle of amplification generates ever increasing amounts of DNA copies. The detection systems Read more »

DNA/RNA PCR Synopsis

Doctor:Correct me if I'm wrong:1. The Qualitative DNA PCR tests for viral DNA within blood cells.2. The Quantitative RNA PCR tests for RNA within the plasma.3. Both will be positive 1 month after exposure.4. The Qualitative DNA PCR will remain...
Mark Holodniy, M.D., F.A.C.P., C.I.C.

Response from Mark Holodniy, M.D., F.A.C.P., C.I.C.

Stanford University, School of Medicine
Great answers. Some qualifications. DNA results will be affected by therapy, and will decline to a lesser extent than plasma HIV RNA. DNA will still be detected in someone with a plasma viral load of MH Read more »
San Francisco AIDS Foundation

Viral Load Testing

...the branched-chain DNA test ( bDNA ), is made by a company called Chiron (pronounced "KAI-ron"). The reverse transcriptase polymerase chain reaction or RT-PCR (commonly called the PCR ) test is made by Roche (pronounced "RO-sh").... Read more »
New York State Department of Health AIDS Institute

Diagnostic, Monitoring and Resistance Laboratory Tests for HIV

...Assays 2. HIV-1 Confirmatory Antibody Assays 3. HIV-2 Antibody Screening B. Viral Identification Assays 1. DNA Polymerase Chain Reaction (DNA-PCR) 2. Plasma HIV RNA Assays III. Monitoring Tests A. Lymphocyte Analysis ... Read more »
The Well Project

Understanding HIV-Related Lab Tests II: Viral Load, Resistance and Tropism

...Viral Load Test Viral load is the amount of HIV in your bloodstream. Viral loadcan be measured by two different lab tests: polymerase chain reaction (PCR) orbranched DNA (bDNA). It is best to stick with the same kind of test and notswitch... Read more »
The Well Project

Women and HIV Viral Load

...of HIV, the greater the risk of your immune system being damaged. Viral load can be measured by two different lab tests: a polymerase chain reaction (PCR) test or a branched DNA (bDNA) test. Both tests are accurate, but each has a different way... Read more »
Connect With Others

DNA PCR Qualitative Tests

Posted by Anonymous, 1 Reply
...Can somebody tell me the accuracy of DNA PCR Qualitative tests? If they are accurate when should they be got done at the earliest? I have read that they are not advised due to high false Read more »

Anyone thinking of taking a PCR please read!

Posted by Anonymous, 4 Replies
...undetectable viral loads despite having HIV infection. Your patient may be one of them." All of us must realize an ELISA at 6 months is the gold standard, and it also proves why experts recommend antibody testing after 2-3 months exposure vs... Read more »

HIV DNA reliability

Posted by Anonymous, 1 Reply dna yet I know will be positive by elisa and am a good case in point. See below. "It has been reported that commercial qualitative HIV proviral DNA tests may not detect all genetic subtypes of HIV. One study reports that although100% of... Read more »

Qualitative PCR

Posted by Anonymous, 1 Reply
...ARS synthomps appeared on the 4th week: pharyngits, fever, swollen nodes, weight loss. 50 days after that exposure I had a Qualitative PCR negative. I know that is not conclusive, but is a good sign. Please I need to know if someone knows about... Read more »