Diagnostic, Monitoring and Resistance Laboratory Tests for HIV
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Table of Contents
|What's New -- June 2010 Update|
Significant revisions include the following:
- Information regarding diagnostic HIV tests has been expanded and updated (see Section II. Diagnostic Tests); such tests include:
- Antibody screening assays
- Home access tests
- Rapid tests
- Western blot for screening oral fluid and urine
- Table 2 has been updated with the latest information regarding available rapid HIV tests
- A table has been added that lists criteria for determining patients who are at risk for HIV-2 infection and should therefore receive combined HIV-1/HIV-2 screening (see Table 3)
- A section on co-receptor tropism detection has been added (see Section III. C. 3. Co-Receptor Tropism Assay)
- The committee now recommends performing HLA-B*5701 testing before initiating abacavir-based therapy (see Section III. D. Human Leukocyte Antigen Testing)
|Key to Abbreviated Terms|
|bDNA||Branched chain DNA|
|CDC||Centers for Disease Control and Prevention|
|ELISA||Enzyme-linked immunosorbent assay|
|FDA||US Food and Drug Administration|
|HLA||Human leukocyte antigen|
|NAT||Nucleic acid amplification test|
|OMT||Oral mucosal transudate|
|PBMC||Peripheral blood mononuclear cell|
|PCR||Polymerase chain reaction|
Technologic advances in HIV laboratory testing continue to aid in screening, diagnosis, and patient management. Effective methods for detection of HIV-related laboratory markers are critical for both accurate HIV screening and for monitoring patients for disease progression and viral resistance to therapy. This chapter provides an overview of currently available HIV laboratory screening methods, viral load assays, and HIV resistance tests. Many new technologies that will further enhance testing methods are in various stages of development.
Patients presenting for testing within 36 hours of exposure to HIV should be assessed for post-exposure prophylaxis (see Post-Exposure Prophylaxis Guidelines). Expert advice may be obtained from the National Clinicians' Consultation Center PEP Line at 1-888-HIV-4911 (1-888-448-4911).
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This article was provided by New York State Department of Health AIDS Institute.
Comment by: Samir Hussain
Sat., Apr. 18, 2015 at 2:31 am EDT
HIV CMIA repeatedly reactive more than 20 months ELFA negative , W. BLOT showed HIV 1 &2 negative but didn't understand the remarks.
vaccinated to inactivated influenza(SANOFI),pneumococcal pcv13 & psv23 (MSD), Hep.B (ENGERIX B) 40 mcg 0,1,2,3,6 after got my first reactive result. I am a medical laboratory technician working for more than 15 years, had flu like symptoms 15 years ago when I first joined lab. Does my elfa will show positive near in future? No Rna PCR done yet. my weight is 82.5 till date, few skin rash in my legs (now very faint) observed 3 years, felling lightheadness, vertigo, left ear infection persists,using DREP(DWD). Please suggest. No ART taken so far.
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