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1997 Revised Guidelines for Performing CD4+ T-Cell Determinations in Persons Infected with Human Immunodeficiency Virus (HIV)

From U.S. Centers for Disease Control and Prevention

APPENDIX. Effects of medications and other biologic factors on immunophenotyping results

Causative agent Effect Result
Zidovudine (AZT) Increased granuloctye fragility; red blood cells (RBCs) less susceptible to lysing reagents Poor light-scattering (increased granulocyte and RBC contamination of lymphocyte gates)
Some antibiotics
(e.g., cephalosporins)		 Increased cellular auto-fluorescence Increased autofluorescence
Corticosteroids Decreased CD4+ T-cell levels Decreased CD4+ T-cell percentage and absolute number
Strenuous exercise Decreased lymphocyte counts Lower absolute lymphocyte subset values
Diurnal variation Changes in absolute lymphocyte counts Variable absolute lymphocyte subset values (not as prevalent in human immunodeficiency virus infection)

*49 CFR parts 100-171 (56 FR 47158).
**42 CFR part 493 § 493.801-493.865.


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This article was provided by U.S. Centers for Disease Control and Prevention. It is a part of the publication Morbidity and Mortality Weekly Report.
 
 

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