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a message for anonymous pc
      #6568 - 07/03/00 11:02 PM

i am the one that posted 1-800 number for the pcr test. according to the counselors at 1-800 number the 28 day dna pcr is conclusive. they told me "sir you do not have it, now you have to convince yourself that you don't". also, over at the hopkins site they break down the accuracy of varios test. the elisa is accurate 99.99 percent of the time and the pcr is listed as being accurate more then 99 percent of the time.

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Re: a message for anonymous pc new
      #6577 - 07/04/00 12:59 AM

Thanks very much for reassuring me, my friend. I've called them as well and got the same response, that accuracy of PCR DNA at 4 weeks is 99.9% however, I took the PCR DNA test at my PCP, one day before you've posted the 800 number, but I guess it's the same test, wherever you go.

A lot of HIV research articles that I've read also mentioned PCR DNA as conclusive at 4 weeks to some degree, with different opininons ranging from 80%-99%, but most of these articles are at least one year or more old. Latest generation of PCR DNA is extremly sensitive, so this is boosting up the estimates a little bit, too.

Good luck with your test results, I'll pray for your NEGATIVE. Let me know when you get it, OK?

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Jedi Master

...yes but.... new
      #6582 - 07/04/00 05:31 AM

even tough negative pcr´s are great news the elisa test still being the gold rule. Pcr are most of the times effective whenever ars is highly suspected but it doesnt work all the time, if that wasnt true then people would not be doing any more elisa test.
So i think to be sure, yes you can have pcr´s done but take an elisa at 3 months.

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Re: ...yes but.... new
      #6587 - 07/04/00 09:01 AM

You're right, Jedy, but my point is that it's highly unlikelly of being infected if tested negative on DNA PCR at 28 days. Dr. M. Holodnyi from Stanford Univ explained in one of the forums about testing and seroconversion that among the test case studies he conducted, he never had anybody who tested negative on a 28 days post exposure DNA PCR to later change serostatus within the following 6 mo.
The same ideea is shared by doctors at Harvard Univ, who ssay that a negative DNA PCR at 28 days is as close as you can get to a medical certainty about not being infected.
I agree that a 3 mo ELISA shoud be done, just to eliminate those 0.01%, 0.1%, 1% uncertainties (nobody knows exactly what the exact figures are).

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Re: ...yes but.... new
      #6613 - 07/04/00 11:42 PM

Another thing to keep in mind is that PCRs are considered extremely accurate when they are negative after 4 weeks. I think most of the innacuracy is due to the fact that they can cause false positives. If it is negative after 4 weeks, I think it's pretty close to the accuracy of the ELISA. But Jedi is right, the gold standard is still the ELISA, so it's pretty good to get retested to be absolutle sure!

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Worried 2000

PCR @ 1 month new
      #6623 - 07/05/00 01:21 AM

The doctors at Harvard University (Rosenberg and Walker) said that the test at one month (and they were talking about getting an RNA and an Elisa in tandem) are "as close as you can get to a medical certainty." They added they would be shocked if a test went from negative to positive later on.

The point is that you are looking for the virus in your blood. How could it not be detected after one month? Maybe, you say, it is too low to be detected (assuming the test measures only down to 400 copies). But if that's the case, then the Elisa should turn positive. Elisa looks for antibodies, which are there only if the virus itself is there. . . . .so if no antibodies, and no virus, then no infection . . . . . .

But you will of course continue worrying because you've been told you need to wait 6 months. . . . . .My point is that when you start getting Elisas after a longer amount of time, the only way that you would be "falsely negative" would be if (1) the PCR test was wrong; (2) the Elisa was wrong). . . . . That is highly unlikely, particularly the longer out you test.

Keep the faith. I know it's difficult, but you must.


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