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HIV Transmission and Education >> Am I Infected?

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Magnus
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How does the HIV test work?
      #32709 - 04/23/02 08:34 AM

ELISA (Enzyme-Linked Immunosorbent Assay) is a other variant of EIA (Enzyme Immuno Assay), this test are in generally the same but diffrent methods used To detect HIV and other viruses. serum from the patient will be mixed with virus components. If the person have antibodies they will bind to the virus proteins. They you will mix this with a other fluid markted(enzyme-linked) and this will bind to s substrate. This will cause a color change then you read out this color change in a spectrometer(or spectrfasemeter) if positive you will move on with western Blot to confirm.



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TerryD
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Re: How does the HIV test work? new
      #32731 - 04/23/02 12:42 PM

It does not bind to "virus proteins" but cellulary debris which can be many things and so the test is what is called non-specific and has no gold standard. This is why it is useless for AIDS testing. It does not test for hiv at all.
Make yourself a wonderfull day!



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