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Cliff
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Reged: 07/03/10
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Posts: 1
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Indeterminate west blot
#251016 - 07/03/10 08:58 AM
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My wife and I just started an IVF cycle in feb of this year. We were both tested for stds and hiv and both tested negative. The first live ivf cycle failed and in April we transferred two of our frozen. The embryos are ours (my sperm, her eggs).
We hit pay dirt and she is pregnant.
Last week we met with our OBGYN for the first time since being released from the fertility dr. Our OBGYN tested my wife for stds and HIV.
Her ELISA was positive and the wb was indeterminate so she ordered a pcr.
Both my wife and I are not in a risk group. We've been married six years and faithful, no drugs, etc, plus we had the negative test results in feb before beginning the ivf.
Results of pcr are not back yet (by the way, how long does that take)?
So, what are your thoughts on the results thus far?
Our fertility nurse tells us non-reactive antibodies are most likely the cause of the positive ELISA and that we are worried for nothing. She says we should expect a negative pcr. Your thoughts on that?
Lastly, if and it's a big if, but if the fertility clinic made an error and transferred an infected embryo, could that explain it?
Thank you for any feedback you have concerning this crisis.
Edited by Cliff (07/03/10 09:14 AM)
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It's quite possible changes in your wife's chemistry because of the pregnancy caused this. Firstly that the ELISA came out reactive, secondly that the WB is indeterminate.
The proper procedure is that another ELISA should be performed in a month. If that one is also positive BUT the WB is again indeterminate then your wife is then also said to be HIV negative. The reason for this is because an indeterminate result shows up for only 2 reasons. 1) The person is in the process of seroconverting. If the person is seroconverting then the WB will be postive when done in a month. 2) Chemistry is screwing with the test results.
While the PCR (viral load) can be useful, it is not approved for diagnosis and since it isn't, if it did come back with a viral load, an ELISA and then a Western Blot would have to be performed in order to confirm the test.
It's purpose is to measure viral load in a patient to see if the medication is working.
There is no way any embryo could be the cause of infection. Children of HIV positive mothers are not at risk UNTIL the day they are born and exposed to their mother's blood. This is why so much care and attention is taken to lower the mother's viral load during the pregnancy and performing c-sections are standard procedure.
Good luck with your baby.
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