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Indeterminate ELISA indeterminate WB scared

Oct 28, 2010

Doctor - Thanks for your reply. My first test result was Postive Elisa and indeterminate WB with P24 strongly reactive. I was really scared after reading everything about P24. I got another ELISA done immediately after 6 days and it came back negative. I am really relieved.As per your advice, I will go for a follow up at the 3 month mark. I am pretty confident that I will be negative due to low risk of oral sex.I have also donated 500$ on your website. Please continue with your good work. Also,please send me your karma so that my final test is negative

Response from Dr. Frascino


A negative follow-up ELISA six days after an indeterminate HIV screening (positive ELISA and indeterminate WB) is conclusive evidence that you are indeed HIV negative!

You are correct that oral sex carries only a very low HIV-acquisition risk. I'm extremely confident your three-month definitive HIV test will remain negative.

Thank you for your very generous gift to The Robert James Frascino AIDS Foundation ( It is both urgently needed and very warmly appreciated. Your one gift will touch many lives in a very meaningful way. On behalf of those lives, please accept my heartfelt thanks. In return I am sending my best good-luck/good-health karma that your conclusive three-month test remains negative and that you are now and will always be HIV free!

Good luck. Many, many thanks!

Dr. Bob

Indeterminate ELISA Indeterminate Western Blot - scared Oct 22, 2010

Hello Dr. Bob - Really appreciate the work with your website.

I am a 30 year old man.30 days before ( Sep 20 2010), I had unprotected oral sex with a female sex worker. She cleaned up my penis before oral with a towel and I am not sure if it had someone else's semen. Also, I ejaculated directly into the same towel with the towel hard-pressed against my penis. There were no other activities.

Similarly, on Oct 6, I had a similar encounter. A sex worker gave me an oral and cleaned me up with a towel before and after. Again, I am not sure if the towel was used on someone else.

I got very scared and went for a HIV test on Oct 15. The Doctor called me today and indicated that results were indeterminate - both ELISA and Western Blot.

I have been very clean all these years and I am really scared now. I am on regular medicine for asthma - Singulair, Advair, Desloratidine and levosalbutamol. Not in the recent past ( 2 - 3 months), but pretty regularly over the years I have taken anotbiotics like Norfloxacin for my stomach upset and IBS. Though I do not remember, my asthma Doctor in India had given me some antigen ( I believe so ) to help with my dust mite allergy and I was on it for an year or so till some time in 2008.

I am really scared. Please let me know what I should do and how to stay positive

Response from Dr. Frascino


Unprotected insertive oral sex carries only a very slight chance for HIV transmission/acquisition. The spunked-up cum towel would not be an HIV risk.

Regarding the test results, I don't understand the indeterminate ELISA. That test is either reactive (positive) or nonreactive (negative). There is no indeterminate ELISA result! If, and only if, the ELISA is reactive (positive), a Western Blot (WB) should be done as a confirmatory test. It can be positive, negative or indeterminate. Your asthma medication from 2-3 months ago would not alter the HIV-antibody test results. Likewise, your dust mite allergy immunotherapy (allergy shots) from 2008 would not affect the validity of your recent HIV-antibody test results.

My advice is to repeat your ELISA (and WB if the ELISA is positive) at the three-month mark (from the date of your last potential exposure). If the test is negative, you are HIV negative. If the test shows a positive ELISA and indeterminate confirmatory WB, you should get an HIV PCR DNA test. This type of test does not rely on anti-HIV antibodies, but rather assays for a piece of the virus's genetic material. It can help sort out inconclusive HIV-antibody test results. If your ELISA and WB are positive, you should see an HIV specialist physician for further evaluation.

I don't think you have to worry about "how to stay positive," because from what you've written, you are most likely "negative"!

Good luck.

Dr. Bob

Positive Elisa, Negative Western Blot, Dr wants to do a HIV Viral Load
Sex and menstrual period with condom

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