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positive EIA, indeterminate Western Blot
Feb 10, 2009

I forgot to include some information. I am a heterosexual female that has had NO unprotected sex in over 1 year (since separating from husband). I've tested negative in 2-3 previous tests but hadn't been tested in a few years. Also, physical exam found that my urine screening indicated an infection that was not identified (possible UTI).

Current test results are: HIV 1/2 EIA AB screen -- REPEATEDLY REACTIVE, see HIV-1 Western Blot Result HIV Antibody, HIV 1, Western Blot -- INDETERMINATE P24 -- REACTIVE P40 -- REACTIVE P55 -- REACTIVE GP160 -- INDETERMINATE HIV 2 AB, EIA, w/REFL WB HIV 2 Antibody, EIA -- NONREACTIVE Doctor said that I may be in early ARS stage, in process of becoming positive (exposure within last 3 months). I'm confused. Is the Elisa (EIA) positive or negative? Is the Elisa (EIA) positive on HIV 1 but negative on HIV 2? Is this a false positive? Could the unidentified infection found in the urine screening have caused reactive results? Please help me understand.

Response from Dr. Frascino

Hello,

HIV-antibody tests consist of two parts: an initial screening test (ELISA, EIA, rapid test) and a confirmatory test (Western Blot or immunofluorescence assay), if the initial screening test is "repeatedly reactive" (positive). This two-part HIV test is not considered to be a "positive HIV test" unless both parts are positive (reactive). Your combined test would be considered "indeterminate." You can read extensively about indeterminate test results in the archives. If indeed you've had no potential HIV-risk exposures over the past year and have tested HIV negative multiple times in the past, your "indeterminate" result will most likely ultimately turn out to be "negative." Your HIV-2 test (a completely different test) is negative. Your abnormal urine test and possible UTI would not affect the results of your HIV tests. At this point your next step is to repeat your EIA (and Western Blot, if the former is positive).

Good luck.

Dr. Bob



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