negative elisa indeterminate western blot
Jan 4, 2008
Your site is excellent. I had a western blot done with out an elisa, western blot was indeterminate p24. then a viral load was done two weeks later which was undetectible, then 6 weeks later I had a negative elisa, equivocal western blot p24, and undetectible viral load, should I do anymore testing. respectfully
Response from Dr. Frascino
Your situation is an excellent example of why Western Blot tests are never recommended as initial HIV-screening tests. They are only used as confirmatory tests after an ELISA test (or other preliminary HIV-antibody screening test) is found to be reactive. So getting a Western Blot without the recommended initial screening test was your first mistake. Your second mistake was running a viral load test (PCR) on your next HIV-screening test, again never having a preliminary HIV-antibody screening test. Your third mistake was running a second Western Blot after getting a negative ELISA result. Who the heck was ordering all these tests in such a helter-skelter fashion for you??? I'd suggest 3 strikes and they are out!!! They certainly weren't doing you any favors. You've wasted a good deal of time, energy, blood, money and unnecessary worry due to improperly ordered tests.
Your negative ELISA test, assuming it was run three months or more after your last potential exposure, is definitive and the only test you needed. All the rest are unwarranted. Your indeterminate Western Blots are falsely reactive at p24. Your two negative viral load tests merely corroborate what we already know from your negative ELISA. Viral load tests are not recommended for routine HIV screening due to the rate of false-positives and cost.
Bottom line: stop testing. You're HIV negative. Next time you need HIV testing, get an ELISA first and follow up with a Western Blot only if the ELISA is reactive, OK?
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