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Orasure Test: Elisia Pos. WesternBlot Pos. Blood Test Elisia Neg., very confused
Nov 6, 2003

Dr. Bob, I took an orasure test in Oct. 2002. Results came back Elisia Pos. and WesternBlot Pos.. Then took three viral load tests within a four month period. All came back undetected. Blood levels all normal. In Feb. 2003 decided to goto Westchester Medical Center in Vahalla NY to take Blood Test. Blood Test came back Elisia NEG!!!! I am very confused. The time I took the first test I had not had sex for 11 months, and have not had sex ever since. I was told by my doctor at WCMC not to worry, but how do you explain the different results. I did have Hodgkins Lymphoma years back, could that have affected the test results? Please answer this...I'm going crazy inside!!!!

Response from Dr. Frascino

Hello,

The Orasure test is a home test using a cotton pad to obtain saliva on which an EIA (enzyme immunoassay) and WB (Western Blot) are run. It generally compares quite well with standard blood tests (99-99.9 percent correlation) Your previous Hodgkin's Lymphoma would not affect the test results. However, you still could be one of the small percentage of people who test positive on Orasure, but are, in reality, seronegative. In addition, there is also the possibility of a technical or clerical error. One of these 2 scenarios is very likely in light of your 3 negative viral load tests and subsequent negative ELISA test. I agree with your WCMC physician: You are HIV-negative. You now have 3 viral loads and an ELISA, all confirming your negative status. The positive Orasure test is the inconsistent finding (a false positive).

Dr. Bob



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