Please Note: Due to volume considerations, not all questions can be answered. Questions most likely to be answered will be those of general interest to a broad group of visitors to this forum. Questions pertaining to a specific case; requests for diagnosis, medical advice, or second opinion; or requests for opinions about untested alternative therapies will generally not be answered.
|
|
 |
 |
science is supposed to be fun, not anxiety provoking!
Apr 6, 2008
Dearest Dr Bob!
Hope you remember the desperate swedish scientist from some years ago.
Well, I am still desperate...
What is even more desperate is that I am still desperate about essentially the same thing,namely the human melanoma cell line that I work with.
It was tested initially,like back in 1972 or something and it came out negative for all kinds of viruses. Since that time many labs have worked with it, incl ours. I guess, no testing since the first one...
Somehow anything I do with this line scares the shit out of me every single time. It is most likely OCD, but I wanted to ask you about some incidents anyway, to get your expert opinion.
1) after the cells of this line sitting on coverslips were fixed in 2% paraformaldehyde for 30 min RT and treated with 0.2% TritonX100 5' RT and washed in PBS, I gently tapped them on Kleenex and after some minutes used that same Kleenex to wipe my nose! stupid! If I did touch the mucous membrane inside my nose, will I be at risk? According to your answers on similar questions,probably not.Eh?
2) While pipetting lysates from these cells (in 0.5% TritonX100) got a very small amount of lysates on my gloves, turned them inside out and checked for leaks by filling them with running H2O; the water poured over on my bare fingers where I had a non-bleeding hangnail. Risk? Hopefully no risk.
I hope it's not too boring to answer my questions, I would really appreciate your input.
Hope the democrats win this time!Will hold fy thumbs for that to happen.
Thanks alot
From Stockholm with love
|
 |
|
 |
|
Response from Dr. Frascino
Hello,
1. HIV risk: nonexistent.
2. HIV risk: nonexistent.
Your worries continue to be completely unwarranted. If you are so concerned about that particular cell line, why not screen it for HIV? The results will undoubtedly be negative and your science will return to being fun instead of anxiety provoking.
Dr. Bob
|
|
|
 Please remember that this forum is designed for educational purposes only, and experts are not engaged through this
forum in rendering legal or medical advice or professional services. Experts appearing on this page are independent and are solely responsible
for editing and fact-checking their material. Neither The Body nor any sponsor is the publisher or speaker of posted visitors' questions or the experts' material.
Questions and messages posted to this forum are not statements of advice, opinion, or information of The Body, Body Health Resources Corporation or any sponsor of this
forum. While neither The Body nor Body Health Resources Corporation regularly reviews posted content, we reserve the right to delete, move, or
edit postings if we deem it appropriate under the circumstances. Visitors submitting questions remain solely responsible for the content of their
messages.
Information provided by experts is general only and should not be used for diagnosing or treating a health problem or a disease, or relied upon as
legal or other professional advice. This information is not a substitute for professional advice or care. If you have or suspect you may have a
health or legal problem, you should consult your own health care provider or your attorney.
Copyright notice.
|
|
|
|
