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really worried took two dna pcr test help
Sep 25, 2011

Im frm india i had high risk exposure with a sex worker in july 2008 i took rapid elisa at5,15,21weeks plus an hiv dna pcr at 5months all neg.second exposure in 2010 oct.again sex worker,.protected,but the condoms is removed afer the climax,in such a way that the vaginal fluid over the condoms came in contact with penis hole.then i took hiv dna pcr in mid december.third exposure is also similar to second exposure on 1st aug 2011.i took hiv dna pcr after 2and5week both not detected plus rapid antibody test by using kit at government hospital in which they giv result 2to5mins at2and4weeks its neg.i didnt rely on that.so i took rapid elisa using immune filtation technique at 45days.its neg my questions are 1)how reliable is hiv1proviral dna by real time pcr.?the report said it follows a combination of nested fluroscent by using thermal cyclic cylinders for incresed sensivity. 2)i took two hiv dna pcr which is done at2and5weeks 3)how reliable is rapid elisa using immune filtration. 4)if some thing is causing symptoms say swollen lymph by hiv will hiv pcr hav the potential to detect.? 5)i've read in the post about a guy that he contracted the hiv on april15 and diagnised positive in early may.will it be possible? 6)can hivpcr giv false negitive? i read false positive is possible but nt false neg.How true is this?i read in us it is possible. 7)how reliable are my test and what percentage u award to my results pls ans do i need further testing.?

Response from Dr. McGowan

The best schedule of testing is to get an ELISA at time of exposure, after 4-6 weeks, 3 months and 6 months. DNA PCR is not recommended for testing of adults, it has use for babies exposed at birth. If a person has symptoms of acute (recent) HIV infection an HIV RNA PCR can be helpful and is a very sensitive test because the amount of virus in the blood after a newly acquired infection is very high. In fact the RNA PCR is more likely to be falsely positive which is why we do not routinely use it to diagnose HIV. Since you had an unsafe sexual encounter in August 1st, it is about 6 weeks, get your ELISA, and repeat in November and Feb 2012.

Take steps to avoid future unsafe exposures by using condoms for sexual activity and don't mix alcohol/drugs with sex.

Best, Joe



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