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DNA PCR - A Confirmatory Test?

Jul 29, 1999

Dr. Holodniy, is a Qualitative DNA PCR considered to be a confirming diagnostic test? If so, who considers it to be confirmatory - the medical community? the FDA? Also, one other question - if one receives a result of "detected", should one repeat the test to rule out any chance of a false positive, or is the test nearly 100% reliable? Thanks so much for sharing your knowledge.

Response from Dr. Holodniy

The Medical community. There are certainly false positives with every test. In studies done in people with HIV antibodies present, DNA PCR is nearly 100% reliable and concordant with the antibody tests. It's a little less reliable in true negatives. MH

3rd day
Is a DNA PCR much more expensive than an RNA PCR and if the answer is yes, why?

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