The Body: The Complete HIV/AIDS Resource
Follow Us Follow Us on Facebook Follow Us on Twitter Download Our App 
Professionals >> Visit The Body PROThe Body en Espanol
Ask the Experts About

Understanding Your LabsUnderstanding Your Labs
Rollover images to visit our other forums!
Recent AnswersAsk a Question
  • Email Email
  • Glossary Glossary

Viral load by PCR versus branch dna

Jan 7, 1999

I am a medical technologist and our lab is about to start testing for hiv by branch DNA. Previously we were sending out our specimens for viral load by PCR to a reference lab. Now we are wondering how do the numbers compare between the two methods? Is there a formula that we can use to convert the numbers so the doctors know how to compare the results. Hope you can give us some help.

Response from Dr. Holodniy

This is a very important question. The current PCR test (HIV-1 Monitor, Roche) with a cutoff of 400 copies/ml, usually gives a viral load value of 2 times greater than the Chiron 2.0 bDNA viral load assay (500 copy/ml cutoff). The new version of the Chiron assay (version 3.0, 50 copy/ml cutoff), gives values about 2 fold greater than version 2.0, or about equal to the current PCR based test. When switching assays you should always rebaseline with 2 assays. MH

Life Insurance HIV Screening.
PCR-DNA Question

  • Email Email
  • Glossary Glossary

 Get Email Notifications When This Forum Updates or Subscribe With RSS



This forum is designed for educational purposes only, and experts are not rendering medical, mental health, legal or other professional advice or services. If you have or suspect you may have a medical, mental health, legal or other problem that requires advice, consult your own caregiver, attorney or other qualified professional.

Experts appearing on this page are independent and are solely responsible for editing and fact-checking their material. Neither nor any advertiser is the publisher or speaker of posted visitors' questions or the experts' material.

Review our complete terms of use and copyright notice.

Powered by ExpertViewpoint