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SmithKline laboratory Report
Feb 15, 1999

HI DC Can youplease explain my dna pcr report was taken 2 and half months a fter contact. Elisa was taken in 12 weeks and was negative please advise also if more testing needed. REad as follows ULTRASENSITIVE HIV-1 PCR QUANTITATION HIV-1 RNA BY PCR QUANT NOT DETECTED COPIES/ML NOT DETECTED{<13 COPIES/ML} HIV-1 RNA COPIES LOG 10 NOT DETECTED LOG 10 NOT DETECTED {<1.1 LOG 10 COPIES/ML} The limit of quantitation of ths assay is26 [i.4 LOG 10] copies of HIV-1 RNA/ML. THE LIMIT OF DETECTION IS 13 [1.1 LOG 10]COPIES OF HIV-1 RNA/ML. SPECIMENCONTAINING GREATER THAN EQUAL TO 13 BUT LESS THAN 26 COPIES /MLARE REPORTED AS DETECTED. SPECIMEN CONTAINING LESS THAN 13 COPIES/ML ARE REPORTED AS NOT DETECTED.

THIS TEST HAS TWOINTENDED USES, FIRST EVALUATING PATIENTS WHO HAS RECEIVED MULTIDRUGG THERAPY FOR HIV-1 INFECTION WHOSE VIRAL LOADS HAVE DECREASED BELOW 500COPIES VIRAL RNA PER ML. SECOND THE TEST HAS RESTRICTED DIAGNOSTIC USE IN DETECTING CIRCULATING VIRAL RNA DURING THE FIRST EFW WEEKS FOLLOWWING POSSIBLE EXPOSURE [WINDOW PERIOD]. THIS TEST IS NOT INTENDED FOR BASELINING PATIENTS PRIOR TOMULTIDRUG THERAPY BECAUSE THE TEST HAS BEEN OPTIMIZED FOR DETECTION OF LOW LEVELS OF CIRCULATING VIRAL RNA.

RAPID PLASMA REAGIN [RPR] NONE REACTIVE NONE REACTIVE CA

PLEASE DR EXPLAIN THANKS VERY MUCH

Response from Dr. Holodniy

The viral load test result you got is from a "qualitative" rather than a quantitative viral load test. This means that the assay has been optimized for a yes/no answer at very low copy number, rather than for giving accurate numbers when monitoring therapy. You don't need further testing. MH



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