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DNA PCR/clades detected?

Jul 1, 2001

Hi Dr. Holodniy,

Concerning HIV1, are groups A, O, N the only clades a DNA PCR will have trouble detecting or are there others?

Response from Dr. Holodniy

The ability to detect all subtypes/clades of HIV is dependent on the design of the PCR assay. Some commercial assays are limited in the clades they will detect. Some reference laboratories have modified the assay to accomodate detection of of most, if not all subtypes.

DNA needed to produce RNA

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