Apr 21, 2013
Why We do not Amplify RNA directly and what is the need to prepare cDNA for amplification of RNA?
Response from Dr. Holodniy
There are some assays that can "amplify" RNA directly, or actually not amplify the RNA itself, but amplify the signal one gets in the assay from a direct measurement of the HIV RNA. A branched DNA assay is one example. Conventional PCR assays use a DNA polymerase to increase the amount of product, hence the need to convert the RNA to DNA first in order to then perform a DNA amplification.
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