Apr 21, 2013
Why We do not Amplify RNA directly and what is the need to prepare cDNA for amplification of RNA?
| Response from Dr. Holodniy
There are some assays that can "amplify" RNA directly, or actually not amplify the RNA itself, but amplify the signal one gets in the assay from a direct measurement of the HIV RNA. A branched DNA assay is one example. Conventional PCR assays use a DNA polymerase to increase the amount of product, hence the need to convert the RNA to DNA first in order to then perform a DNA amplification.
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