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HIV 1 QUANTITATIVE REAL TIME PCR
Feb 26, 2012

HELLO Doctor, i did a HIV-1 quantitative real time PCR which says the result is viral copies / ml is less than detectable limit(<100). what does that mean? does this test amplify viral RNA or DNA or the virus number itself? If it quantifies RNA/DNA, then why they say the result in terms of viral copies/ml instead of RNA copies or DNA copies /ml? my last question: in few articles i saw the reference lowest level for this test is <40 copies/ml, then why this lab detects only copy number >100? regards.

Response from Dr. Holodniy

There are various PCR assays for HIV viral load quantitation, and they can have different lower limits of detection. The assays detect viral RNA copy number, but by virtue of the technology, PCR is actually detecting the viral RNA that has been converted to DNA in the assay.



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