Jul 1, 2007
My question is...If some viral loads are in the 100,000s, would it be possible to isolate HIV from such an infected individual using his/her fresh uncultured blood plasma instead of using PCR to detect the HIV? Would we find whole retrovirus at 1.16 gm/ml level?
Thanks for your answer.
P.S. I think you're HOT!
Response from Dr. Holodniy
Not sure what you mean by isolate HIV. There are other molecular techniques that can detect and quantify plasma HIV RNA not using PCR. They all require some form of an amplification scheme, either by boosting the signal or amplifying the nucleic acid first prior to detection. Virus can be cultured from the plasma, but that has it's own inherent sensitivity issues and can't be cultured from everybody. I don't think there is enough virus to quantitate by protein means, certainly not at the level you describe.
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