|Difficult (but important) question about different techniques to sequence the virus
Dec 2, 2006
I understand Brian Conway's team (University of British Columbia) picked up an atypical case of HIV by sending a plasma sample for resistance test, even though the patient had negative ELISAs 12 and 14 months after exposure, negative bDNA, negative DNA PCR and a negative co-culture with PBMCs. See http://www.thebody.com/Forums/AIDS/SideEffects/Archive/Testing/Q156332.html. Brian Conway,M.D. explains that as part of this process, the resistance test sequences the virus using a technique that is quite different from Roche PCR and bDNA.
My very basic understanding is that PCR technology is used in the viral load assay and the genotypic resistance test. However the resistance test then requires further technology to determine the DNA sequence of the PCR product that was generated.
I would be FOREVER GRATEFUL if you would advise me how precisely/else the resistance test is different from Roche PCR and bDNA.
| Response from Dr. Holodniy
This is too complex of a question to answer here. You are correct that PCR is used in the first step of the genotyping resistance assay followed by a sequencing reaction to determine the DNA sequence that uses different biochemical steps. PCR is not used for the bDNA assay.
Get Email Notifications When This Forum Updates or Subscribe With RSS
- How Is Hiv Different From Other Viruses?
- Hepatitis C Test Is Negative Can I Still Have It
- Acute Retroviral Syndrome Symptoms
- Is Lexapro Used To Treat Peripheral Neuropathy?
- Nortriptyline And Neuropathy
- Can Fluid Buildup Around Eyes And Blurred Vision Be Caused By A Viral Infection Or Hiv?
This forum is designed for educational purposes only, and experts are not rendering medical, mental health, legal or other professional advice or services. If you have or suspect you may have a medical, mental health, legal or other problem that requires advice, consult your own caregiver, attorney or other qualified professional.
Experts appearing on this page are independent and are solely responsible for editing and fact-checking their material. Neither TheBody.com nor any advertiser is the publisher or speaker of posted visitors' questions or the experts' material.