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Confusing: so many different types of primers in EIA tests

Dec 5, 2003

Dr. Holodniy, What are the most sensitive and broadly reactive (for all HIV-1 subtypes) primers for an ELISA test?... I have read conflicting information, some say recombinant proteins, others say whole viral lysate and others still say synthetic peptides.

I would appreciate your clarification.

Thank you.

Response from Dr. Holodniy

Primers are NOT used in EIA tests. EIA tests utilize proteins or antigens to detect antibodies. Primers are small pieces of DNA that are used in molecular testing to detect HIV RNA or DNA in a sample, NOT antibodies.

primo infection
labs and flu shot

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