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bDNA vs pcr
Sep 22, 2003

Hi, For years I went to a Quest LAb where they used bDna vl testing.Now, I use a doc at the VA Hosp and they use thepcr 1.0. MY understanding is that 1,000 copies on a bDna test are about = to 2,000 copies on the pcr test.Is that right? Also,when we read guidelines about when to start or restart treatment and they discuss the vl and don't mention if its a bDna or a pcr, which one are they referring to? Thank you,Luke

Response from Dr. Holodniy

The majority of studies were performed using PCR. With the current version of Amplicor HIV Monitor PCR 1.5 and the Versant bDNA 3.0, the viral loads are pretty comparable. However, guidelines mention both assays. There is also a third approved assay technology called NASBA. When treatment decisions are considered there is not enough difference between assay results, that one would make radically different treatment decisions based on different assays.



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