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bDNA and PCR
Aug 2, 2003

Hi, I read an archived post about the diff betw bDNA and PCR vl testing.YOu said that the PCR could be 2-3 times higher than the bDNA and you need to establish a new baseline. Could you help to understand my test results.?

4/05 21,000 bDna 5/06 19,400 bDNA 6/03 23,000 bDNA 7/02 23,663 Amplicor 1.0 pcr 7/07 18,400 bDNA

How would you convert the pcr to the bDNA and how would you interpret theses results Thanks

Response from Dr. Holodniy

I would say in your case, that your viral load levels are quite stable regardless of which technology is being used. Establishing a new baseline is only important if you are changing to a new assay technology and will stick with the new one. The difference in viral load between these two technologies, on average, yields a PCR result about 1.5 times more than bDNA. Since this is an average, many people will have identical levels, which is the case for you.



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