Different Viral load Test made me get confused
Feb 16, 2003
Mi question is this: Does is make any difference in between the techniques which quantifies HIV- RNA such as AMPLICOR, NUCLISENS(NASBA) and those that uses HIV-DNA such as BDNA-BAYER I am asking this because Ive got a RNA VL below the limit of detection <50 copies and then Ive got a result of 2758 copies using the other technique HIV- BDNA BAYER. Could you please tell me what is the difference and which one is more reliable to monitor HAART. THANKS A LOT F.L.P
Response from Dr. Holodniy
The differences between these technologies is about 2 fold or 2 times the actual value. In general, bDNA (Versant) results are about two time less than Amplicor or Nuclisens. The difference you cite, would be significantly different, and well outside the correction factor for assay comparison. It is advisable to stick with one assay type for consistent information.
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