Advertisement
The Body: The Complete HIV/AIDS Resource
Follow Us Follow Us on Facebook Follow Us on Twitter Download Our App
Professionals >> Visit The Body PROThe Body en Espanol
Read Now: TheBodyPRO.com Covers AIDS 2014
   
Ask the Experts About

Understanding Your LabsUnderstanding Your Labs
           
Rollover images to visit our other forums!
Recent AnswersAsk a Question
  
  • Email Email
  • Glossary Glossary


quantifying viral load
Oct 5, 2000

Is viral load determined before or after PCR amplification? The reason I ask is that I see "50 perMl" given as the number at which the virus is undetectable, and that would mean that before PCR virtually everyone who is HIV positive is "undetectable".

Response from Dr. Holodniy

A 50 copy viral load is what is there before PCR. An internal standard of known copy number is added to each patients sample of unknown copy number. Based on the signal obtained in a PCR assay for the known standard, the patient copy number is calculated. After PCR there are literally billions of copies of HIV floating around. We could never detect 50 copies without the amplification that PCR affords. So a viral load of 50 copies is a back extrapolation to the starting material.

MH



Previous
CD test
Next
Stopped Meds

  
  • Email Email
  • Glossary Glossary

 Get Email Notifications When This Forum Updates or Subscribe With RSS


 
Advertisement



Q&A TERMS OF USE

This forum is designed for educational purposes only, and experts are not rendering medical, mental health, legal or other professional advice or services. If you have or suspect you may have a medical, mental health, legal or other problem that requires advice, consult your own caregiver, attorney or other qualified professional.

Experts appearing on this page are independent and are solely responsible for editing and fact-checking their material. Neither TheBody.com nor any advertiser is the publisher or speaker of posted visitors' questions or the experts' material.

Review our complete terms of use and copyright notice.

Powered by ExpertViewpoint

Advertisement